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The last proposed mechanism of RBC vasodilation during the presence of nitrite could be the reduction of nitrite to NO by Hb. Brooks first analyzed the nitrite Hb interaction in 1937 which do the job was later extended by Doyle in 1981 (Brooks, 1937; Doyle et al., 1981). Nitrite reacts with deoxygenated Hb to sort metHb and NO (Brooks, 1937; Doyle